Bio-Rad Bio-Plex System Validation and Calibration Tools User Manual
Page 19
12. Repeat procedure if values are not within the specification range. If values
are again not within acceptable specification ranges, contact Bio-Rad
technical support for assistance.
10.5 Validation of Classify Efficiency
Procedure
1.
If not already done, follow the procedure for start-up and calibration of the
Bio-Plex system.
N
No
otte
e:: B
Be
e s
su
urre
e tto
o c
ca
alliib
brra
atte
e iim
mm
me
ed
diia
atte
elly
y b
be
effo
orre
e v
va
alliid
da
attiio
on
n.. IIff y
yo
ou
u a
arre
e u
us
siin
ng
g B
Biio
o--P
Plle
ex
x
M
Ma
an
na
ag
ge
err 4
4..0
0//4
4..1
1,, u
us
se
e e
eiitth
he
err tth
he
e h
hiig
gh
h o
orr llo
ow
w R
RP
P1
1 tta
arrg
ge
ett ffo
orr C
Ca
all2
2 c
ca
alliib
brra
attiio
on
n
d
de
ep
pe
en
nd
diin
ng
g o
on
n tth
he
e tty
yp
pe
e o
off a
as
ss
sa
ay
y b
be
eiin
ng
g p
pe
errffo
orrm
me
ed
d.. IIff y
yo
ou
u a
arre
e u
us
siin
ng
g B
Biio
o--P
Plle
ex
x
M
Ma
an
na
ag
ge
err 5
5..0
0,, tth
he
e c
ca
alliib
brra
attiio
on
n iis
s d
do
on
ne
e o
on
n llo
ow
w R
RP
P1
1 tta
arrg
ge
ett.. S
Se
elle
ec
cttiio
on
n o
off h
hiig
gh
h R
RP
P1
1
tta
arrg
ge
ett iis
s d
do
on
ne
e iin
n tth
he
e v
va
alliid
da
attiio
on
n s
sc
crre
ee
en
n..
2.
Remove the classify validation bead set from 4°C storage and vortex each
bottle for 30 sec.
3.
Place 5 drops of each classify bead into the corresponding classify well
labeled as A, B, C, D, and E in the MCV plate.
4.
Store stock vials at 4°C as soon as possible after use. Protect beads from
light.
5.
Select IIn
ns
sttrru
um
me
en
ntt from the main menu. Select V
Va
alliid
da
attiio
on
n from the pull-down
menu.
6.
Enter the User name and select control number. Select C
Clla
as
ss
siiffy
y V
Va
alliid
da
attiio
on
n.
Select OK. The following dialog box will appear (Figure 10):
Fig. 10. Classify validation dialog.
7.
Select the Eject button to eject the plate holder.
8.
Place the MCV plate on the microplate platform.
9.
Select O
OK
K to start the classify validation procedure.
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