Bio-Rad Bio-Plex System Validation and Calibration Tools - Manual (Page 14)

12. Check the results for each validation. If specifications are not met for any
validation, repeat that validation. If the value again is not within the
specification range, contact Bio-Rad technical support for assistance.
13. The following sections describe the steps for performing the individual
validations (optics, fluidics, reporter, or classify).
14. Validation results are also logged into a validation log in Bio-Plex Manager. To
access this log, select V Viie ew w from the main menu then V Va alliid da attiio on n L Lo og g. Each
type of validation is stored in a separate log for the purpose of tracking data
over time. See the Bio-Plex Manager user manual for your software version
for more information on using the validation log.
10.2 Validation of Optics Alignment
Procedure
1. If not already done, follow the procedure for start-up and calibration of the
Bio-Plex system.
N No otte e:: B Be e s su urre e tto o c ca alliib brra atte e iim mm me ed diia atte elly y b be effo orre e v va alliid da attiio on n.. IIff y yo ou u a arre e u us siin ng g B Biio o--P Plle ex x
M Ma an na ag ge err 4 4..0 0//4 4..1 1,, u us se e e eiitth he err tth he e h hiig gh h o orr llo ow w R RP P1 1 tta arrg ge ett ffo orr C Ca all2 2 c ca alliib brra attiio on n
d de ep pe en nd diin ng g o on n tth he e tty yp pe e o off a as ss sa ay y b be eiin ng g p pe errffo orrm me ed d.. IIff y yo ou u a arre e u us siin ng g B Biio o--P Plle ex x
M Ma an na ag ge err 5 5..0 0,, tth he e c ca alliib brra attiio on n iis s d do on ne e o on n llo ow w R RP P1 1 tta arrg ge ett.. S Se elle ec cttiio on n o off h hiig gh h R RP P1 1
tta arrg ge ett iis s d do on ne e iin n tth he e v va alliid da attiio on n s sc crre ee en n..
2. Remove the optics validation bead set from 4°C storage and vortex each
bottle for 30 sec.
3. Place 5 drops each of optics beads 1 and 2 into the respective wells on the
MCV plate.
4. Store optics beads at 4°C as soon as possible after use. Protect the beads
from light.
5. In Bio-Plex Manager software, select IIn ns sttrru um me en ntt from the main menu. Select
V Va alliid da attiio on n from the pull-down menu.
6. Enter user name and control number. Select O Op pttiic cs s. Select O OK K. The
following dialog appears (Figure 4):
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