Bio-Rad Bio-Plex System Validation and Calibration Tools - Manual (Page 32)

Bio-Plex Manager 2.0 and MCV Plate Instructions
Performed by:
I. Optical Validation
DD median 4774–6593
CL1 median 3383–4135
CL1 CV% 3–7%
CL2 median 3520–4302
CL2 CV% 4–8%
RP1 median 15205–18583
RP1 CV% 5–10%
II. Reporter Validation
B(Blank)
1
2
3
4
5
III. Classify Validation
Region 34 >80%
Region 38 >80%
Region 54 >80%
Region 73 >80%
Region 77 >80%
IV. Validation of Fluidics Integrity
Fluidics Integrity Validated? (Place “X” in appropriate box)
To Generate Report:
1. Open Excel and open the ValidationReport.xls file.
2. Click on 'Worksheet' tab and enter values from this worksheet.
3. Click on Report and select 'File' then 'Print' to print report.
Protein Array Reader
Validation Kit Worksheet
4106178 Rev A
Parameter Specification Measured Value
Region Specification Measured Value
YES NO
Bead Median Channel
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