Bio-Rad Bio-Plex System Validation and Calibration Tools - Manual (Page 30)

2. Select the Unclog icon from the toolbar.
3. The following dialog box appears:
Fig. 19. Unclog dialog.
4. Add 5 drops of CAL1 beads to the CAL1 well on the MCV plate.
5. Fill the DI H
2
O and 70% isopropanol reservoirs.
6. Select the Eject button in the dialog box to eject the plate holder.
7. Place the MCV plate in the Bio-Plex microplate platform.
8. Select OK to begin the validation of fluidics integrity procedure.
9. If the unclog procedure was successful, the following message will appear:
10. Check "yes" in Section IV of the validation kit worksheet.
11. If the procedure is unsuccessful, the following message will appear: If this is
the case, repeat steps 4–11.
12. If the procedure is again unsuccessful, contact Bio-Rad technical support for
assistance.
Bio-Plex Manager 2.0 and MCV Plate Instructions
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