Bio-Rad Bio-Plex System Validation and Calibration Tools - Manual (Page 19)

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9
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Fig. 9. Reporter validation results.
11. The results will also be logged into a validation log in Bio-Plex Manager. See
Bio-Plex Manager user manual for more information on using the validation log.
12. Repeat procedure if values are not within specifications. If any values are
again not within acceptable ranges, contact Bio-Rad technical service for
assistance.
13. Acceptable reporter validation specifications:
Parameter Specification
Dynamic Range 4.15–4.28
Linearity >0.995
Slope
0.0593–0.0799
Accuracy >90%
Sensitivity
<200 MESF
9.5 Validation of Classify Efficiency
Procedure
1. If not already done, follow the procedure for start-up and calibration of the
Bio-Plex system.
2. Remove the classify validation bead set from 4°C storage and vortex each
bottle for 30 sec.
3. Place 5 drops of each classify bead into the corresponding classify well
labeled as 34, 38, 54, 73, and 77 in the MCV plate II (see Figure 2).
4. Store stock vials at 4°C as soon as possible after use. Protect beads from
light.
Bio-Plex Manager 3.0 and MCV Plate II Instructions
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