Bio-Rad Bio-Plex System Validation and Calibration Tools - Manual (Page 26)

Fig. 14. Optics validation results.
14. Acceptable optics validation specifications:
Parameter Specification
DD median See insert
CL1 median See insert
CL1 CV% 3–7%
CL2 median See insert
CL2 CV% 4–8%
RP1 median See insert
RP1 CV% 5–10%
If any results are not within the acceptable specifications, please contact
Bio-Rad technical service.
If any values do meet specification, repeat the procedure. If values are again
not within specifications, contact Bio-Rad technical service for assistance.
10.2 Validation of Reporter Channel Performance
Procedure
1. If not already done, follow the procedure for start-up and calibration of the
Bio-Plex system. Note: Be sure to calibrate immediately before validation.
Use the High RP1 Target value for CAL2 calibration.
2. Remove the reporter validation bead set from 4°C storage and vortex each
bottle for 30 sec.
Bio-Plex Manager 2.0 and MCV Plate Instructions
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