Bio-Rad Bio-Plex System Validation and Calibration Tools - Manual (Page 28)

11. Record the median channel values in a copy of the worksheet provided with
the validation kit.
12. Repeat procedure if values are not within specifications. If any values are again
not within acceptable ranges, contact Bio-Rad technical service for assistance.
13. Acceptable reporter validation specifications:
Parameter Specification
Dynamic Range 4.15–4.28
Linearity >0.995
Slope
0.0593–0.0799
Accuracy >90%
Sensitivity
<200 MESF
10.3 Validation of Classify Efficiency
Procedure
1. If not already done, follow the procedure for start-up and calibration of the
Bio-Plex system. Note: Be sure to calibrate immediately before validation. Use
the High RP1 Target value for CAL2 calibration.
2. Remove the classify validation bead set from 4°C storage and vortex each
bottle for 30 sec.
3. Place 5 drops of each classify bead into the corresponding classify well labeled
as 34, 38, 54, 73, and 77 in the MCV plate (see Figure 2).
4. Store stock vials at 4°C as soon as possible after use. Protect beads from
light.
5. Select Instrument from the main menu. Select Validation from the pull-down
menu. Select Classify from the pop-up menu. The following dialog box
appears:
Fig. 17. Classify validation dialog.
6. Select the Eject button to eject the plate holder.
Bio-Plex Manager 2.0 and MCV Plate Instructions
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