Bio-Rad Bio-Plex System Validation and Calibration Tools - Manual (Page 29)

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7. Place the MCV plate on the Bio-Plex microplate platform.
8. Select OK to start the classify validation procedure.
9. When the procedure is completed, values will be displayed in a dialog box as
shown below:
Fig. 18. Classify Validation dialog.
10. Record the classify efficiency values in a copy of the validation kit worksheet
provided in this manual.
11. Acceptable classify efficiency values:
Classify Bead Specification
34 >80.0%
38 >80.0%
54 >80.0%
73 >80.0%
77 >80.0%
Note: If any values do not meet specifications, repeat the procedure. If
values are again not within specifications, contact Bio-Rad technical service
for assistance.
10.4 Validation of Fluidics Integrity
Procedure
1. If not already done, follow the procedure for start-up and calibration of the
Bio-Plex system. Note: Be sure to calibrate immediately before validation.
Use the High RP1 Target value for CAL2 calibration.
2. Remove the calibration bead set from 4°C storage and vortex each bottle for
30 sec.
Bio-Plex Manager 2.0 and MCV Plate Instructions
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