Bio-Rad Bio-Plex System Validation and Calibration Tools - Manual (Page 27)

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3. Place 5 drops of each reporter bead into the corresponding reporter well
labeled as B (blank), 1, 2, 3, 4, and 5 in the MCV plate (see Figure 2).
4. Store reporter beads at 4°C as soon as possible after use. Protect the beads
from light.
5. Fill the DI H
2
O reservoir with water.
6. Select Instrument from the main menu. Select Validation from the pull-down
menu. A dialog box will appear. Select Reporter from the pop-up menu. The
following dialog box appears:
Fig. 15. Reporter validation dialog.
7. Select the Eject icon in the dialog box to eject the plate holder.
8. Place the MCV plate in the Bio-Plex microplate platform.
9. Select OK to start the reporter validation procedure.
10. When the procedure is completed, values will be displayed in a dialog box as
shown below:
Fig. 16. Reporter validation results.
Bio-Plex Manager 2.0 and MCV Plate Instructions
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