Bio-Rad Bio-Plex System Validation and Calibration Tools - Manual (Page 3)

Table of Contents
Section 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Section 2 Product Description . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
Section 3 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
Section 4 Storage and Handling . . . . . . . . . . . . . . . . . . . . . . . . . . .3
Section 5 Principle of Optics Validation . . . . . . . . . . . . . . . . . . . .4
Section 6 Principle of Reporter Channel Validation . . . . . . . . . . .4
Section 7 Principle of Classify Validation . . . . . . . . . . . . . . . . . . .7
Section 8 Principle of Fluidics Validation . . . . . . . . . . . . . . . . . .8
Section 9 Procedure for Bio-Plex Manager 3.0/MCV plate II . . .9
9.1 One-Step Procedure for all Validation Parameters . . . . . . . . .9
9.2 Validation of Optics Alignment . . . . . . . . . . . . . . . . . . . . . .11
9.3 Validation of Fluidics Integrity . . . . . . . . . . . . . . . . . . . . . . .13
9.4 Validation of Reporter Channel Performance . . . . . . . . . . . .15
9.5 Validation of Classify Efficiency . . . . . . . . . . . . . . . . . . . . . .16
9.6 Generating a Validation Report . . . . . . . . . . . . . . . . . . . . . .18
9.7 Validation Report Example . . . . . . . . . . . . . . . . . . . . . . . . .19
Section 10 Procedure for Bio-Plex Manager 2.0/MCV plate . . . .21
10.1 Validation of Optics Alignment . . . . . . . . . . . . . . . . . . . . . .21
10.2 Validation of Reporter Channel Performance . . . . . . . . . . . .23
10.3 Validation of Classify Efficiency . . . . . . . . . . . . . . . . . . . . . .25
10.4 Validation of Fluidics Integrity . . . . . . . . . . . . . . . . . . . . . . .26
10.5 Generating a Validation Report . . . . . . . . . . . . . . . . . . . . . .28
10.6 Validation Kit Worksheet and Report
Form Examples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
Section 11 Troubleshooting Guide . . . . . . . . . . . . . . . . . . . . . . . . .32
Section 12 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . .32
Section 13 Reference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
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