Bio-Rad Bio-Plex System Validation and Calibration Tools User Manual
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Section 9
Procedure for Bio-Plex Manager 3.0 and
MCV plate II
Introduction
This section provides instructions for use of the Bio-Plex Validation Kit 3.0 with
Bio-Plex Manager version 3.0 and MCV plate II. Bio-Plex Manager now provides
a fully automated validation routine that sequentially performs all the validation
tests without further user intervention. To perform all validations in a single step,
follow instructions in Section 9.1. If you wish to perform an individual validation
routine, these instructions are provided in Sections 9.2–9.5.
9.1 One-Step Procedure for all Validation Parameters
Procedure
1.
Turn on the Bio-Plex array reader, microplate platform, and computer as
specified in the Bio-Plex hardware and Bio-Plex Manager user manuals.
2.
Perform start-up procedure as directed.
3.
Calibrate Bio-Plex array reader using CAL 1 and CAL2 beads found in the
Bio-Plex Calibration Kit according to the Bio-Plex Manager software manual.
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4.
Remove all validation bead sets from 4°C storage and vortex each and every
bottle for 30 sec. This is very important for proper validation.
Bio-Plex Manager 3.0 and MCV Plate II Instructions
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