Bio-Rad Gel Doc™ XR+ System User Manual
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Please follow the procedure in the table to ensure that the Universal Hood II is functioning properly.
STEP 1. Initial test for the Universal Hood II:
Procedure
Control Panel
Make sure the door and drawer are closed and the computer is switched on.
Turn on the system
Power LED turns on after short blinking.
Wait 30 minutes for camera to warm up
Nothing will change except the camera will warm up.
Press the Epi White key
Epi-white LED turns on.
Open the door
Epi-lights are on, Trans UV LED blinks.
Press the Epi White key again
Close the door
Epi-lights and LED are turned off.
Trans UV LED turns off.
Press the Trans UV key
Trans UV LED turns on.
Open the door
Trans UV LED blinks.
Close the door
Trans UV LED turns off.
Press the Trans UV key
Trans UV LED turns on.
Open the drawer half way
Trans UV LED blinks.
Close the drawer
Trans UV LED turns off.
Press the Trans UV key
Trans UV LED turns on.
Press the Prep UV key
Prep UV LED turns on.
Press the Hold key
Hold LED turns on.
Open the drawer
Trans UV LED blinks, Prep UV LED and
Hold LED turns off.
Close the drawer
Trans UV LED turns off.
Note: The light source buttons on the control panel are disabled when the Imaging LED blinks
(indicating that the computer is switched off).
STEP 2. Procedure for initialization of the camera and lens assembly both (for GelDoc XR/
ChemiDoc XRS systems)
1. Press the epi iIllumination button.
2. Open the door and determine if lights are on.
3. Place the focusing target on the UV transilluminator.
4. Double click on Quantity One software icon on the desktop. From File Menu, select Gel Doc XR
or ChemiDoc XRS.
Note: “Dark Reference” comes up on first use for both GelDoc XR system. Please refer to the step 3
for details about reference file image acquisition.
5. Select Live/Focus. This applies to Chemi-Doc XRS System only, Gel Doc XR does this
automatically.
6. An image of the target will appear on the computer screen. Using the lens control buttons on the
membrane touch pad or the control panel that appears in the software, find the best Iris, Focus, and
Zoom conditions.
Note: Working with a widely open Iris and changing the Zoom may require you to adjust the Focus.