Bio-Rad CHEF-DR II System - Manual (Page 9)

Model 1000 Mini Chiller
The Model 1000 Mini Chiller is a stand alone, portable refrigerated apparatus specifi-
cally designed for use with the CHEF-DR II system. Electrophoresis buffer is circulated by
the variable speed pump directly through the unique heat exchanger, which is a tube within
a tube. Buffer circulates through the inner stainless steel tube, while liquid refrigerant circu-
lates through the outer copper tube, resulting in rapid and efficient cooling at a rate of
0.75 °C/minute (from ambient temperature to 14 °C). The external temperature probe regu-
lates cooling by the Model 1000 Mini Chiller, resulting in precise maintenance of buffer tem-
perature in the electrophoresis cell.
Section 2
Operation
2.1 Instrument Setup
Place the CHEF-DR II electrophoresis cell on a level surface, with the control module on
top of the drive module and to the right or on a shelf above. The electrophoresis cell should
be positioned with the T connector facing you and the safety interlock to your right. If the
system includes a Model 1000 Mini Chiller, place it to the left of the cell. Place the variable
speed pump at the rear of the cell and connect the plug from the pump to the port labeled
PUMP CONNECTOR in the front of the drive module. Level the electrophoresis cell with the
four leveling feet at each corner of the electrophoresis cell by placing the leveling bubble
(provided) in the center of the base of the cell.
Fig. 2.1. Interconnections between components of the CHEF-DR II system.
Attach the power cords for the control module, drive module, and Model 1000 Mini
Chiller in the back of each instrument. Be sure that both the control module and drive mod-
ule are powered off. Connect the 25-pin cable from the electrophoresis cell to the port labeled
OUTPUT TO ELECTROPHORESIS CELL on the front panel of the drive module. Connect
the coiled interlock cable from the electrophoresis cell to the jacks labeled TO INTERLOCK
on the drive module.
Electrophoresis
Chamber
CHEF-DR II
Power Module
Variable Speed
Pump
Mini-Chiller
IN
OUT
25 Pin Cable
3/8"Tygon Tubing
Safety Interlock Cable
CHEF-DR II
Control Module
- +
- +
A+ B+
A+ B+
From Power
Supply From
Pulsewave
6