Bio-Rad CHEF-DR II System - Manual (Page 10)

Connect the set of black and red cables from the left set of jacks on the control module labeled
POWER SUPPLY (- and +) to the left set of jacks on the drive module labeled FROM POWER
SUPPLY. Connect - to - with the black cable, and + to + with the red cable. Note: the metal
plugs at the ends of these cables have smaller diameters.
Connect the set of red cables from the right set of jacks on the control module labeled
A+ and B+ to the right set of jacks on the drive module labeled FROM PULSEWAVE.
Connect A+ to A+ and B+ to B+.
2.2 Electrophoresis Cell Operation
When using the Model 1000 Mini Chiller, attach approximately 1-2 feet of 1⁄4 inch inter-
nal diameter Tygon tubing to both the FLOW IN and FLOW OUT ports on the Model 1000
Mini Chiller, and secure the tubing with plastic clamps (provided). Connect 2 feet of 3⁄8 inch
internal diameter Tygon tubing directly from the left port of the T connector to the 1⁄4 inch
tubing from the FLOW IN of the Model 1000 Mini Chiller using a 3⁄8 to 1⁄4 inch reducer
(provided). The pump should be positioned between the outlet of the Model 1000 Mini Chiller
and the inlet of the electrophoresis cell. Connect the 1⁄4 inch tubing from the FLOW OUT of
the Model 1000 Mini Chiller to the inlet of the CHEF-DR II pump using another 3⁄8 to 1⁄4 inch
reducer. Connect approximately one to two feet of Tygon tubing from the outlet of the pump
to the single connector at the rear of the electrophoresis cell using a 3⁄8 inch connector (pro-
vided). When using a general purpose, recirculating water bath as a chiller, attach about 9.5
feet of 3⁄8 inch internal diameter Tygon tubing to the left outlet port of the T connector on the
electrophoresis cell. Connect the other end of the Tygon tubing to the inlet of the CHEF-DR
II pump. Connect two feet of Tygon tubing from the outlet of the pump to the single con-
nector at the rear of the electrophoresis cell. Coil approximately 4-5 feet of the longer tubing
within the bath of the chiller.
Finally, connect a six inch piece of Tygon tubing to the right outlet port of the T con-
nector and clamp the tubing securely closed; this will be used to drain the buffer in the elec-
trophoresis cell.
Insert the gel frame into the positioning holes in the electrophoresis cell. There are two sets
of three positioning holes. Place the frame only in the holes at the bottom corners of the gel,
opposite the wells, so that the frame is centered in the cell. The outermost holes are for the
optional 21 x 14 cm (8.25 x 5 inch) gel format (See casting stand Instruction Manual for more
details).
If the system includes the Model 1000 Mini Chiller, connect the temperature probe to
the port labeled REMOTE SENSOR on the front panel of the Model 1000 Mini Chiller. Insert
the temperature probe into the lid of the electrophoresis cell. Remove the temperature probe
when opening the lid to prevent breakage and for easy access to the electrophoresis cell.
It is desirable to establish the correct buffer flow prior to attempting any electrophoresis
runs. Once the desired flow rate has been achieved, subsequent electrophoresis runs should
be with the same setting on the variable speed pump. Fill the chamber with 2 liters of the
buffer of choice. The desired flow rate of buffer through the electrophoresis cell is approxi-
mately 0.75 liter per minute (approximately 70 on the variable speed pump). Turn on the
pump and measure the flow of buffer at the drain port by removing the clamp from the 6”
piece of tubing. Make adjustments to the buffer flow with the variable speed pump.
It is also very beneficial to fine tune the chiller before attempting any electrophoresis
runs. Turn on the chiller and pump approximately 1⁄2 hour before adjusting the temperature.
Initially, it will be necessary to fine tune the temperature setting to achieve a consistent run-
ning temperature.
7