Bio-Rad CHEF-DR II System - Manual (Page 32)

5. Hansenula wingei
Size Range: 1-3.1 mb
Agarose: 0.8% Molecular Biology Certified
Buffer: 1.0x TAE
Temperature: 14 °C
Switch Time: 250-900 seconds
Run Time: 50 hours
Voltage Gradient: 3 V/cm
6. Schizosaccharomyces pombe
Size Range: 3.5-5.7 mb
Agarose: 0.8% Chromosomal Grade
Buffer: 1.0x TAE
Temperature: 14 °C
Switch Time: 1800 seconds
Run Time: 72 hours
Voltage Gradient: 2 V/cm
Section 6
Maintenance of Equipment
6.1 Replacing Electrodes
The electrophoresis cell requires little maintenance except for rinsing after every run.
Dirt and other build-up can be removed with laboratory detergent and a fine cloth. Care should
be taken not to bend or break the electrodes.
Fast switch times (<2 seconds) with high voltage gradients (6 V/cm) may lead to increased
electrode failure If one of the electrodes should break, or leak at the O-ring, it may be replaced.
Additional electrodes (Catalog number 170-3646) are available from Bio-Rad Laboratories.
Thick electrodes (0.02”; Catalog number 170-4648) which are very resistant to the breakage
associated with high voltage, short switch time run conditions, are also available.
To replace an electrode, turn the electrophoresis cell upside down and remove the six
screws. Lift off the base plate. Remove the hexagonal nut on the wire of the broken electrode,
then remove the hexagonal nut on the electrode to be replaced. Push down firmly on the post
to remove the old electrode. Turn the box over, insert the new electrode, pack with self-lev-
eling silicone sealant (RTV-type silicone sealer available at most hardware stores), and replace
the nut. Replace the wire and base plate.
If one of the pins to the serial cable bends, use tweezers to carefully straighten it.
Replacement cables may be ordered from Bio-Rad.
29