Bio-Rad CHEF-DR II System - Manual (Page 13)

Volts/cm
This will display the set voltage gradient for 3 seconds after which the remaining time
for the current Block in progress will be displayed.
Actual Current
This will display the actual current for the Block in progress. The BLOCK and RUN
TIME indicator lights will be illuminated. If the power supply is at current limit (500 mA
maximum), the BLOCK and RUN TIME indicator lights will flash.
Pause/Start Run
Initially, this will initiate the program, and the actual current (in mA) will be displayed
with the BLOCK, RUN TIME, and START/PAUSE indicator lights illuminated. After 5 sec-
onds, the remaining run time (hours) will be displayed with the RUN TIME and
START/PAUSE inidicator lights illuminated. While the program is running, pressing this
key will put the program into PAUSE and the actual current will be displayed with the BLOCK
and RUN TIME indicator lights illuminated, while the START/PAUSE indicator light will
flash. Pressing this key again will resume the program, and the actual current will be dis-
played for 5 seconds.
In a muti-block program it is possible to examine the run parameters of any Block that is
not currently being displayed by pausing the CHEF-DR II system. While in PAUSE, the
Raise and Lower keys are active to scroll through the two Blocks. Any parameter in either
Block may be displayed by pressing the appropriate key. Editing of run parameters is not
possible once the program has started.
Pausing the Program
The program in progress can be paused to examine set run parameters by pressing
START/PAUSE while the unit is running. When START/PAUSE is pressed, the actual cur-
rent will be displayed, with the BLOCK and RUN TIME indicator lights illuminated, while
the START/PAUSE indicator light will flash. Warning: There are high voltages and cur-
rents within the chamber, which can be harmful. Wait for the displayed current to reach zero
before removing the lid of the electrophoresis cell. To resume the program, press
START/PAUSE, and actual current will be displayed for 5 seconds, after which the remain-
ing time for the current Block in progress will be displayed.
Clearing the Program
It is possible to completely clear every parameter in Blocks 1 and 2 of a current program
to the default settings. This can be done turning off the control module. Individual parameters
may be reset to defaults by pressing RAISE and LOWER simultaneously for 6 seconds (it will
beep once per second).
Program Termination
The program in progress may be manually terminated by holding down START/PAUSE
for three to four seconds. A program can only be terminated while it is in the RUN mode, it
can not be terminated in the PAUSE mode. When the program is terminated it will beep twice
and the unit will display OFF. Pressing START/PAUSE again will start the program from the
beginning.
When the program terminates under the timer control, the RUN TIME and
START/PAUSE indicator lights will go off, it will sound 2 beeps per second for 10 seconds,
and the unit will display End. The run timers will be reset and all parameters will be retained.
The run parameters may be used again as is, or further modified, and the program may be
started again by pressing START/PAUSE.
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