Bio-Rad CHEF-DR II System - Manual (Page 11)

2.3 CHEF-DR II Operation
This section describes general operation. See Sections 3 and 4 for sample preparation,
gel casting, gel running, and staining. In the following sections capitalized words refer to
keys, modes, or indicator lights on the CHEF-DR II control module (e.g. RUN TIME) while
boldface words refer to messages on the display (e.g. End).
Power Up
The CHEF-DR II front panel display is shown in Figure 2.2. The front panel consists of
a three segment display, BLOCK, VOLTS/CM, RUN TIME, START/PAUSE, INITIAL
SWITCH TIME, FINAL SWITCH TIME, CURRENT, and RAISE and LOWER keys. On
power up, the unit will beep once and display b-1 with the BLOCK indicator light illuminat-
ed, indicating Block 1 is active. The program parameters will be at the default settings.
Fig. 2.2. Front Panel display of the CHEF-DR II system.
Parameter Entry
The CHEF-DR II system has the flexibility of using up to two separate, consecutively
executing Blocks. Each Block has the run parameters of Initial Switch Time, Final Switch
Time, Run Time, and Volts/cm. During a run, Block 1 is run first and then Block 2. To start
entering the run parameters, turn on the control module. The unit will beep once and display
b-1 with the BLOCK indicator light illuminated, indicating Block 1. If not, press LOWER until
b-1 is displayed. Press VOLTS/CM (the VOLTS/CM indicator light will be illuminated), and
enter the desired value with RAISE and LOWER. Press RUN TIME (the RUN TIME indi-
cator light will be illuminated), and enter the desired value with RAISE and LOWER. Press
BLOCK and VOLTS/CM simultaneously (both the BLOCK and VOLTS/CM indicator lights
will be illuminated), and set Initial Switch Time with RAISE and LOWER. Press VOLTS/CM
and RUN TIME simultaneously (both the VOLTS/CM and RUN TIME indicator lights will
be illuminated), and set Final Switch Time with RAISE and LOWER. If more than 1 Block
is desired, press BLOCK and go to Block 2 by pressing RAISE. The unit will display b-2
with the BLOCK indicator light illuminated, indicating Block 2. Continue entering the run
parameters as in Block 1. Below are the limits for each of the run parameters.
8