Bio-Rad CHEF-DR II System - Manual (Page 21)

Example Calculations:
Mammalian:
(A)
(B)
(C)
(D)
Bacterial:
(A)
(B)
(C)
(D)
18
(6 x 10 9 bp)(660 g/mole) = 6.547 x 10 -12 g DNA/cell
6.02 x 10 23 bp/mole
(6.578 x 10 -12 g/cell)(5 x 10 7 cells/ml) =
(3.289 x 10 -4 g DNA/ml)(1x10 6 µg/g) - 329 mg DNA/ml
(329 µg DNA/ml)(1.2 genome equivalents)
≅
(394 µg DNA/ml)
394 µg DNA/ml ≅ 40 µg DNA/plug ≅
20 µg DNA/lanes
10 plugs/ml 2 lanes/plug
(4.5 x 10 6 bp)(660 g/mole)
= 4.933 x 10 -15 g DNA/cell
6.02 x 10 23 bp/mole
(4.933 x 10 -15 g DNA/cell)(5 x 10 8 cells/ml) =
(2.467 x 10 -6 g DNA/ml)(1x10 6 µg/g) = 2.5 µg DNA/ml
(2.5 µg DNA/ml)(2.5 genome equivalents)
≅
(6.25 µg DNA/ml)
6.25 µg DNA/ml
≅
0.625 µg DNA/plug
≅
0.3 µg DNA/lane
10 plugs/ml 2 lanes/plug