Bio-Rad Silver Stain Plus Kit User Manual
Page 8
6 Silver Stain Plus
3. Staining and Developing Step — 20 min
Staining Solution Preparation and Procedure
(prepare within 5 min of use)
Place 35 ml deionized water into a large
beaker or Erlenmeyer flask and stir with
a PTFE-coated stir bar. Add the following to the
beaker in this order:
5.0 ml Silver Complex Solution
5.0 ml Reduction Moderator Solution
5.0 ml Image Development Reagent
Immediately before use quickly add 50 ml of the
room temperature development accelerator solution
to the beaker. Swirl well. Add the contents of the
beaker to the staining vessel. Stain with gentle
agitation.
Stain both mini and large-format gels for
approximately 20 min or until desired staining intensity
is reached. It may take at least 15 min before the
bands first become visible. Note: Staining time is
dependent on the sample and the quantity loaded.
After the desired staining is reached place the gels in
5% acetic acid to stop the reaction.
4. Stop Step — 15 min
Prepare a 5% acetic acid solution to stop the staining
reaction.
Place gels in stop solution for a minimum of 15 min.
After stopping the reaction rinse the gels in high-purity
water for 5 min. The gels are then ready to be dried or
photographed.