Bio-Rad Silver Stain Plus Kit - Manual (Page 14)

12 Silver Stain Plus
Troubleshooting Guide (continued)
Problem Cause Solution
Bands are
taking longer
than 20 min
to develop
and a high
background
is developing
with the
extended
staining time. Since all proteins
are not identical
longer fixative
times may be
required in some
cases. Extend the fix step
15–30 min to
improve detection.
Note: The fixative step is
a desirable stopping
point if the staining
procedure cannot be
completed in one day.
The presence of
acetic acid still in
the gel from the
fixative step will
slow detection. Extend the wash times.
The development
accelerator
solution was cold
when used. Allow the development
accelerator solution
to come to ambient
temperature before use.
If you have further question about the procedure, call technical
service in the U.S. at 1-800-424-6723 or contact
your local Bio-Rad office.