Bio-Rad Silver Stain Plus Kit - Manual (Page 11)

Silver Stain Plus 9
Place the sealing gasket over the gel sandwich and apply
vacuum. Do not apply heat. High heat will melt the gel. Dry
overnight. When dry, remove the cellophane from the gel
and follow the procedure for polyacrylamide gel staining.
Silver Reagent Disposal
The final staining solution is considered hazardous
waste and should not be poured down the sink. Place
the staining solution in a disposable plastic or hazardous
waste container under the hood. In 1–2 days the silver
metal will precipitate from the solution. Once the silver has
precipitated the aqueous waste can then be decanted
from the silver and disposed of separately. Disposal of
both the solid and aqueous waste should be according to
local and state regulations.
Silver Staining after Coomassie Blue
R-250
Completely destain the Coomassie stained gel in
40% methanol/10% acetic acid. Follow with the wash step
of the Silver Stain Plus procedure and thoroughly rinse the
destain from the gel. Proceed with the staining step.
Destaining
Gels stained with Silver Stain Plus can be completely
destained in 1% hydrogen peroxide. Use several changes
of 100 ml of 1% hydrogen peroxide per mini gel for
2–15 min or until completely destained. To restain, place
gel in fixative enhancer solution (step 1) or 10% acetic
acid. Water wash (step 2) and proceed with staining.