Bio-Rad Silver Stain Plus Kit - Manual (Page 4)

2 Silver Stain Plus
Additional Materials Needed
In addition to the components in the Silver Stain Plus kit,
the following materials are required:
• Reagent-grade methanol and acetic acid
• Deionized, distilled, or high-purity water
• Water of 18-megohm-cm resistivity recommended
• Polytetrafluoroethylene (PTFE)-coated stir bars
• Glass or plastic staining trays
All steps in this procedure require gentle agitation of the
gel in solution. For best results, a shaker table is
recommended.
Warnings and Precautions
Read each label in the kit before beginning the procedure.
Wear gloves, safety glasses, and protective clothing while
preparing and working with all the solutions in this kit.
The image development reagent should be used only in
areas with good ventilation. Avoid breathing vapors. Avoid
contact with skin. In case of contact with eyes, flush with
copious amounts of water and contact a physician.
See Material Safety Data Sheets for additional
information.
Storage
All reagents except the development accelerator solution
can be stored at room temperature. The development
accelerator solution should be stored at 4°C but allowed
to reach ambient temperature before use. This may be
done by aliquoting the volume needed and either warming
in a water bath for 2–5 min or allowing to stand at room
temperature for approximately 30 min before use.