Bio-Rad Silver Stain Plus Kit - Manual (Page 13)

Silver Stain Plus 11
Troubleshooting Guide
Problem Cause Solution
The silver
precipitates
on the staining
containers. Staining
containers may
contain residues
from soap or
prior staining
solutions, which
silver will bind
with to form
metallic silver. Silver staining techniques
require containers to be
thoroughly cleaned before
use. After washing
containers with laboratory
soap and water, rinse
with 50% nitric acid
solution to remove
residues. Water rinse the
acid from the container.
Use deionized distilled
water as a final rinse.
The staining
solution is
cloudy or
contains a
precipitate.
Note: The
presence of
a precipitate
does not affect
the quality of
staining but may
require longer
staining times. The development
accelerator
solution is too
dilute. Add 2–10 ml of
development accelerator
solution until the
precipitate disappears.
If the precipitate does not
disappear, the solution
may still be used, but
precipitate should be
prevented by following
these tips.
1. The development
accelerator solution
must be used at
ambient temperature.
Precipitate will form
if the solution is used
cold.
2. Always add the
aliquoted volume
of development
accelerator solution
all at once and never
a few ml at a time.