Bio-Rad Silver Stain Plus Kit - Manual (Page 5)

Silver Stain Plus 3
Recommendations for Optimal
Staining
• To prevent silver deposits on staining trays and
inconsistent staining, all containers used for mixing
and staining should be scrupulously clean. Glass,
polyethylene, or polypropylene containers may be
cleaned with 50% (approx. 8 N) nitric acid after
cleaning with laboratory detergent. Rinse thoroughly
with high-quality deionized water
• Throughout the procedure the gel should always be
completely submerged in solution. Gels that float on
the surface of the solution will not stain consistently
and will show background discoloration
• Avoid staining gels in direct sunlight or at
temperatures above 25°C
• Never touch gels with metal objects or bare skin.
PVC or latex gloves rinsed with deionized water
should be used if the gel must be handled. Perform
gel manipulations with glass or polyethylene rods, if
possible
• Stopping point: The fixative step is a desirable
stopping point if there is not enough time to complete
the procedure