Bio-Rad Silver Stain Plus Kit - Manual (Page 6)

4 Silver Stain Plus
Reagent Preparation
Development Accelerator Solution
1. Prepare once for use with the entire contents of the
kit.
2. Place 950 ml of deionized distilled water in a
1 L cylinder containing a PTFE-coated stir bar.
3. Begin stirring and slowly add the entire
50 g contents of the development accelerator reagent.
Stir until dissolved.
4. Add water to make 1 L. Pour the solution into the
provided empty 1 L bottle labeled development
accelerator solution.
5. Store at 4°C. Use at room temperature.
If the entire solution will not be used within 3 months,
prepare the development accelerator solution according to
your use rate.
To prepare half the solution, add 25 g development
accelerator reagent to 475 ml deionized distilled water,
add water to make 500 ml.