Bio-Rad Silver Stain Plus Kit - Manual (Page 7)

Silver Stain Plus 5
Polyacrylamide Gel Staining Procedure
The following preparations are adequate for staining two
mini gels (8 x 10 cm), 0.75–1.0 mm thick. Refer to Table A
for staining gels of other sizes.
1. Fixative Step — 20 min
Fixative enhancer solution
preparation (for 2 mini gels)
Reagent-Grade
Methanol 200 ml 50% V/V
Reagent-Grade
Acetic Acid 40 ml 10% V/V
Fixative Enhancer
Concentrate 40 ml 10% V/V
Deionized Distilled
Water 120 ml 30% V/V
Total 400 ml 100% V/V
After gel electrophoresis, place gels in the fixative
enhancer solution. With gentle agitation fix the gels for
20 min. Refer to Table A for fixing times and solution
volumes for larger gels.
2. Rinse Step — 20 min
Decant the fixative enhancer solution from the staining
vessel. Rinse the gels in 400 ml deionized distilled
water for 10 min with gentle agitation. After
10 min, decant water and replace with fresh rinse
water. Rinse for an additional 10 min. Decant rinse
water. For larger and thicker gels, rinse with 800 ml
water for 40 min.