Bio-Rad PDS-1000 / He™ and Hepta™ Systems - Manual (Page 49)

8.3 Specifications
General Specifications, PDS-1000/He Biolistic Particle Delivery System
Dimensions 29 (width) x 25.5 (depth) x 47.5 (height) cm
Construction Aluminum, ABS plastic and acrylic chassis
Weight 15 kg
Electrical
input voltage
100-120 VAC, 50- 60 Hz
Maximum current <5 Amps
Mechanical
Fuse 6.3 A, 250 V, 5 x 20 mm
Vacuum <0.4 inches Mercury/minute leakage
Over-Pressure 0.5 psi relief valve, self-resetting
Environmental
Operating 32 °F (0 °C) to 95 °F (35 °C) temperature, 0-95%
non-condensing humidity
Storage 32 °F (0 °C) to 158 °F (70 °C) temperature, 0-95%
non-condensing humidity
Sole Source Specifications, PDS-1000/He Biolistic Particle Delivery System
The following are the sole source specifications for the Biolistic PDS-1000/He unit:
1. The system is covered by patents numbers 161807, 670771, 877619, 074652, IP-0844,
07/303/503, 07/529989, 437848, 4945050, 5036006, 5100792, and 621561 together with
all continuations, divisionals, and continuations-in-part applications, any patents that issue
on applications and reissues thereof. The Biolistic PDS-1000/He instrument is available for
sale or lease accordingly. Biolistic is a registered trademark of E. I. du Pont de Nemours
and Company. The Biolistic technology is exclusively licensed to Bio-Rad Laboratories.
2. A system capable of generating high pressure gas delivery with a mechanism for deliv-
ering an instantaneous cold gas shock wave into an enclosure where the gas shock is
released, contained and vented.
3. The unit contains a throat region in the high pressure gas delivery system that allows for
interchangeable inserts (rupture disks) which translates the cold gas shock into acceleration
of microprojectiles coated with biological molecules for delivery into diverse target cells/tis-
sue without killing the cells and/or tissue. Nine interchangeable disks are available in 450, 650,
900, 1,100, 1,350, 1,550, 1,800, 2,000 and 2,200 pounds per square inch (psi) pressure.
4. Employs a method for launching the biologically coated microcarriers from the target
side of a planar surface of a flexible plastic sheet that is positioned to be accelerated by
the gas shock wave such that it will move freely in the direction of the target cells until
restrained by a barrier. The barrier permits the microcarriers to move toward the target cells
and also effectively baffles the gas shock wave to deflect some of the force of the cold gas
shock wave away from the target cells with sufficient acceleration of microprojectiles
coated with biological molecules for delivery into diverse target cells/tissue without killing
the cells and/or tissue.
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