Bio-Rad PDS-1000 / He™ and Hepta™ Systems - Manual (Page 20)

Section 4
Operation of the PDS-1000/He Instrument
4.1 Preparation of System Components Prior to Bombardment
Instrument Preparation
1. Verify that helium tank has 200 psi in excess of desired rupture disk pressure for bom-
bardment.
2. Set gap distance between rupture disk retaining cap and microcarrier launch assembly.
When the rupture disk retaining cap is in place, insert the fully assembled microcarrier
launch assembly (with cover lid) inside the bombardment chamber on the highest possi-
ble bombardment chamber wall slot (Figure 4.1). The set screw in the white plastic shelf
should face outward. Release the set screw on the front of the microcarrier launch assem-
bly with the smaller of the two hex key wrenches provided (Figure 2.4).
Fig. 4.1. Microcarrier launch assembly and target shelf inside bombardment chamber.
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