Bio-Rad PDS-1000 / He™ and Hepta™ Systems - Manual (Page 3)

Table of Contents
Section 1 Biolistic PDS-1000/He Particle Delivery System ..............................................1
1.1 Particle Delivery Technology ....................................................................................1
1.2 Overview of PDS-1000/He Particle Delivery System ..............................................1
1.3 Important Safety Information.....................................................................................3
1.4 Requirements for System Operation..........................................................................3
Section 2 Product Description.....................................................................................5
2.1 Packing List................................................................................................................5
2.2 Identification of Unit Controls and Components ....................................................10
Section 3 Installation ..................................................................................................13
3.1 Connecting the PDS-1000/He System to a Helium Source ....................................13
3.2 Connecting the PDS-1000/He to a Vacuum Source................................................15
3.3 Power Cord/Voltage Regulator................................................................................16
Section 4 Operation of the PDS-1000/He Instrument ............................................17
4.1 Preparation of System Components Prior to Bombardment...................................17
4.2 Performing a Bombardment.....................................................................................22
4.3 Removal of Residual Helium Pressure–Shut Down ...............................................30
Section 5 Selection and Adjustment of System Bombardment Parameters........30
5.1 Overview -Matrix of Variables: Cell Types, Settings and Conditions ...................30
5.2 Vacuum Level in Bombardment Chamber..............................................................31
5.3 Helium Pressure / Rupture Disk Selection ..............................................................31
5.4 Solenoid Valve Adjustment .....................................................................................32
5.5 Vacuum Flow Rate Control Valves.........................................................................32
5.6 Distance Between Rupture Disk and Macrocarrier.................................................32
5.7 Distance Between Macrocarrier and Stopping Screen............................................32
5.8 Distance Between Stopping Screen and Target Shelf
(microcarrier flight distance)....................................................................................32
5.9 Microcarrier Selection..............................................................................................33
5.10 Preparation of Biological Material for Bombardment ............................................34
Section 6 Troubleshooting......................................................................................................37
6.1 Rupture Disk Bursts at Incorrect Pressure...............................................................37
6.2 Stopping Screen Forced Through Screen Support Ring .........................................37
6.3 Excessive Gas Usage................................................................................................38
6.4 Chamber Will Not Hold Vacuum............................................................................38
6.5 Sample Damage From Gas Pressure Wave.............................................................38
6.6 Unit Will Not Pressurize Gas Acceleration Tube....................................................39
Section 7 Product Information..................................................................................40
7.1 Biolistic System........................................................................................................40
7.2 Spare Parts................................................................................................................41
Section 8 Appendices ..............................................................................................................43
8.1 Cleaning the PDS-1000/He Device .........................................................................43
8.2 Metal Case Version..................................................................................................43
8.3 Specifications ...........................................................................................................46
8.4 Performing a Bombardment—Quick Guide Tear-Out............................................47