Bio-Rad PDS-1000 / He™ and Hepta™ Systems - Manual (Page 4)

Section 1
Introduction to Particle Delivery
1.1 Particle Delivery Technology
Biolistic particle delivery is a method of transformation that uses helium pressure to intro-
duce DNA-coated microcarriers into cells. Microprojectile bombardment can transform such
diverse targets as bacterial, fungal, insect, plant, and animal cells and intracellular organelles.
Particle delivery is a convenient method for transforming intact cells in culture since minimal
pre- or post-bombardment manipulation is necessary. In addition, this technique is much eas-
ier and faster to perform than the tedious task of micro-injection. Both stable and transient
transformation are possible with the Biolistic particle delivery system.
1.2 Overview of PDS-1000/He Particle Delivery System
The Biolistic System
The Biolistic PDS-1000/He instrument uses pressurized helium to accelerate sub-cellu-
lar sized microprojectiles coated with DNA (or other biological material) over a range of
velocities necessary to optimally transform many different cell types. The system consists of
the bombardment chamber (main unit), connective tubing for attachment to vacuum source,
and all components necessary for attachment and delivery of high pressure helium to the main
unit (helium regulator, solenoid valve, and connective tubing).
Fig. 1.1. Unit components, front view.
1
Helium
Pressure
Regulator
Vacuum (reinforced PVC)
Tygon Tubing
2.5 ft PEEK Tubing
6 ft PEEK Tubing
Tank of Pressurized Helium
(user provided)
Rupture Disk
Retaining Cap
3-Way Helium Metering
(solenoid) valve
Microcarrier Launch
Assembly
Helium Regulator
Target Plate Shelf