Bio-Rad PDS-1000 / He™ and Hepta™ Systems User Manual
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Fig. 2.9. Front view of PDS-1000/He unit.
Table 2.2. Front View-Interior of Bombardment Chamber
Bombardment Chamber
Closes chamber with a solid piece of polycarbon-
Door (with Brace)
ate plastic. Note the single, large o-ring which
seals vacuum in the chamber, and the self-posi-
tioning brace which eliminates flex of chamber
walls during bombardment cycle.
Rupture Disk
Seals Rupture Disk against chamber end of Gas
Retaining Cap
Acceleration Tube. This must be tightened securely.
The Torque Wrench is used in the holes in the cap
which are visible in the photo.
Microcarrier Launch Assembly
Holds the DNA/microcarrier preparation on a
Macrocarrier sheet over the Stopping Screen in
the path of the helium shock wave.
Target Shelf
Holds the biological target in a Petri plate in the
path of the accelerated DNA/microcarrier prepara-
tion. Particle flight distance is determined by posi-
tioning the shelf at one of four levels using slots in
the chamber walls.
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Microcarrier
Launch Assembly
Power Switch
ON/OFF
Helium Pressure Gauge
Target Shelf
Bombardment
Chamber Door
Vacuum Gauge
Vac/Vent/Hold Switch
Vacuum/Vent Rate
Control Valves
Fire Switch
Disk Retaining
Cap