Metrohm 930 Compact IC Flex ChS/Deg - Manual (Page 90)

5.14 Separation column
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
82 ￭￭￭￭￭￭￭￭
930 Compact IC Flex ChS/Deg (2.930.1260)
Checking the rinsing time
You can determine if the applied rinsing time is sufficient by measuring
the sample carry-over directly. Proceed as follows to do this:
1 Preparing two samples
￭ Sample A: A typical sample for the application.
￭ Sample B: Ultrapure water.
2 Determining "Sample A"
Allow "Sample A" to run through the sample path for the duration of
the rinsing time; inject and then measure it.
3 Determining "Sample B"
Allow "Sample B" to run through the sample path for the duration of
the rinsing time; inject and then measure it.
4 Calculating the sample carry-over
The sample carry-over corresponds to the ratio of the peak areas of
the measurement from Sample B to the measurement from Sample
A. The smaller this ratio is, the smaller the sample carry-over. This
ratio can be changed by varying the rinsing time. This can be used to
determine the required rinsing time for the application.
5.14 Separation column
5.14.1 Separating efficiency
The analysis quality that can be attained depends in a large part on the
separating efficiency of the separation column being used. The separation
efficiency of the selected separation column must be sufficient for the cur-
rent analysis problems. If difficulties arise, start by checking the quality of
the separation column in each case by recording a standard chromato-
graph.
You can find detailed information on the separation columns available
from Metrohm in the leaflet provided along with your separation column,
in the Metrohm IC Column Program (available from your Metrohm
representative) or on the Internet at
http://www.metrohm.com in the Ion
Chromatography product area. You can find information on special IC
applications in the corresponding "Application Bulletins" or "Applica-
tion Notes", which are available on the Internet at
http://
www.metrohm.com in the Applications area or free of charge upon
request from your responsible Metrohm representative.