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5 working with the vt1000 s on a daily basis, Working with the vt1000 s on a daily basis, Operation – Leica Biosystems VT1000 S User Manual

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Instructions for Use, V 2.0 RevD – 09/2013

5. Operation

5.5 Working with the VT1000 S on a daily basis

• Mount the buffer tray (1) onto the bolt (2) inside the cooling bath (3).

• Secure the buffer tray by relocating the clamping lever (4) to the right (in

the direction of the arrow).

• Via the UP/DOWN toggle switch lower the buffer tray to its lowest posi-

tion (indicated by audible signal and red LED).

• Move the toggle switch back to the mid-position - the audible signal stops.

• If necessary, fill crushed ice into the cooling bath (3).

• Fill the buffer tray (1) with cooled buffer solution.

• Fix the specimen onto the specimen disc with cyanoacrylate adhesive

(Fig. 24).

• Insert the specimen disc (5) with the specimen into the buffer tray using

the manipulator (6).

• Use the manipulator (8) to rotate the specimen disc into the desired posi-

tion. Tighten with a No. 3 Allen key (7).

• The clamping screw or one of the clamping devices must not be located

over the gap in the specimen disc, as in these positions clamping the

specimen disc is not possible.

• Remove the manipulator (8).

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2

3

4

5

6

7

8

Fig. 23

Fig. 24

Fig. 25

Fig. 26