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Introduction – Techne TC-5000 User Manual

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INTRODUCTION

The TC-5000 is a gradient thermal cycler, designed to accurately control the temperatures of samples
between 4°C and 99°C. It has many scientific applications including DNA amplification, PCR

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, reverse

transcription and sequencing.

The TC-5000 is programmed via a touch screen LCD graphics display. The user is able to input various
programs and parameters, including:

• Specified temperatures in °C
• Times for which the sample is to be held at the specified temperature
• Heating and cooling rates between the specified temperatures in °C/sec
• Gradient of temperatures across the thermal cycling block
• Incremental time or temperature increases or decreases when a cycle is repeated

Before using the TC-5000 make sure you have read this manual carefully. If there is any doubt relating
to the proper use of this equipment, the staff at Bibby Scientific Ltd or your supplier will be happy to
assist you.

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Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser's own internal research and

development and applied fields other than human in-vitro diagnostics under non-real-time thermal cycler patents of Applied Biosystems LLC.

TC

-5000