Techne TC-5000 - Manual (Page 26)

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A heated lid mat (Part No. 6104978) is supplied with the TC-5000; this is for use with 96-well PCR
plates sealed with an adhesive seal. The rubber mat should be placed in top of the sealing film to
ensure good thermal contact with the heated lid. Do not over-tighten the lid as this will cause the
silicone rubber membrane to deform and will reduce the effectiveness of the heated lid.
ADJUSTABLE SCREEN
The brightness of the screen can be adjusted according to the light conditions in the laboratory.
The brightness control is located under the front cover of the instrument. After lifting the front cover
the small black knob in the centre is rotated clockwise to make the screen brighter and anticlockwise
to make it darker.
CONSUMABLES
Bibby Scientific Ltd does not recommend any specific tube or reaction vessel other than those described
in this manual. The tubes must be able to withstand a pressure of 1 atmosphere at 100°C in addition
to the temperatures being used in the thermal cycler block. The tube must not deform to the point of
fracture.
We recommend using reaction volumes between 10µl and 50µl. To test the tube, put 25µl of water in
each of 5 tubes and subject them to a typical program. At the end of the program measure the volume
using a pipette. A loss of more than 1-2µl indicates sample evaporation and a vapour leak. The
volume lost and the change in reagent concentrations determines the minimum volume that can be
used. Typical volume losses of 1µl in 30 cycles allow the use of samples of 10µl or less.
TC-
5000