Luminex xPONENT 4.0 SP1 User Manual
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Table of Contents
Introduction .......................................................................................................................... 1
Software Packages ......................................................................................................... 1
About This Guide ............................................................................................................ 2
Warnings, Notes and Symbols........................................................................................ 2
General Guidelines ......................................................................................................... 3
Biological Samples .................................................................................................... 3
Bead Handling ........................................................................................................... 3
Limitations ............................................................................................................ 3
Safety Precautions ............................................................................................... 4
Bead Concentration................................................................................................... 4
Repetitive xMAP Bead Measurements...................................................................... 4
Classification and Reporter Fluorochromes .............................................................. 4
Sample Volume ......................................................................................................... 5
Sample Dilution ......................................................................................................... 5
Reagents ................................................................................................................... 6
Gating ........................................................................................................................ 6
Plates......................................................................................................................... 6
Touring the Software............................................................................................................ 7
Log In .............................................................................................................................. 7
Help................................................................................................................................. 8
Log Off ............................................................................................................................ 8
Exit .................................................................................................................................. 8
Screen Elements............................................................................................................. 9
System Monitor ............................................................................................................. 10
Home ............................................................................................................................ 12
Samples ........................................................................................................................ 13
Samples Tab ........................................................................................................... 14
Create Sample Tab ................................................................................................. 15
Batches ......................................................................................................................... 16
Batches Tab ............................................................................................................ 16
Create a New Batch from an existing Protocol........................................................ 18
Protocol Tab....................................................................................................... 18
Stds & Ctrls Tab ................................................................................................. 19
Plate Layout Tab ................................................................................................ 21
Create a New Batch From a New Protocol.............................................................. 24
Settings Tab ....................................................................................................... 24
Analytes Tab ...................................................................................................... 27
Stds & Ctrls Tab ................................................................................................. 31
Plate Layout Tab ................................................................................................ 32
Create a New MultiBatch......................................................................................... 33
Results .......................................................................................................................... 37