Luminex xPONENT 4.0 SP1 User Manual
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Luminex xPONENT 4.0 Software Manual
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Quantitative - Determines the sample concentrations from standard curves using
regression methods Cubic Spline, Linear, Logistic 4P, and
Logistic 5P. Type the
desired values for standards and controls in the Number of Standards and Number
of Controls boxes. Select either Fit of All Standards or Mean of Replicates for the
calculation of the curve fit.
NOTE:
Luminex recommends Fit of All Standards as the most accurate
calculation of the curve fit.
Based on a range of quantitative, numerical results, a threshold range can be applied
to a quantitative analysis, for example, high, low, saturated, and expected.
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Allele Call - Sets analysis for an allele call. Analytes must put in groups of 2, 3, or 4
analytes.
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Min MFI Enabled - Select this box to enable a minimum MFI for the Allele Call
analysis. Type a value in this box to set the minimum MFI for analysis.
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Number of Standards - Click to type the number of standards for the protocol.
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Number of Controls - Click to type the number of controls for the protocol.
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Fit of all Standards - The standard curve will be determined by using each individual
standard replicate when calculating the standard curve. For example if one runs
duplicates of a 7 point standard curve, the software will calculate the standard curve by
using 14 points.
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Mean of Replicates - The standard curve will be determined by averaging the
individual standard replicates when calculating the standard curve. For example if one
runs duplicates of a 7-point standard curve, the software will calculate the standard
curve by using 7 averaged points.
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Analyze Results While Acquiring Samples - The software allows for real-time
viewing of the results as the instrument analyzes samples.
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Use External Analysis Program - Select this check-box to use a third-party program
to analyze the data. The Analysis Program list becomes active when this is selected.
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Analysis Program - Use this list to select which program to use for data analysis.
Cancel. Click to return to the main Batches tab.
Next. Click to advance to the Analytes tab.