Luminex xPONENT 4.0 SP1 - Manual (Page 145)

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Glossary
DD temperature The current temperature of the doublet discriminator avalanche photo
diode.
dot plot Graphical representation of microsphere analysis classification data
displayed by frequency and intensity.
doublet discriminator (DD) A classification channel measuring side scatter; used to filter for single
bead events based on size.
emission spectrum Wavelength range that an excited fluorochrome emits when its
electrons fall from a higher to a lower energy state. Expressed in
nanometers (nm).
event Occurs when the signal processor determines that a particle of any
size has passed through the lasers.
excitation spectrum Wavelength range that excites a molecule’s electrons to a higher
energy state. Expressed in nanometers (nm).
fluorescence Light emission that occurs when the electrons of a fluorochrome drop
to a lower energy state.
fluorochrome A fluorescent molecule.
fluorophore See fluorochrome.
gate A user-defined parameter in the histogram represented by red dotted
lines on the graph and numerically indicated in parentheses in the
lower left corner of the graph. The DD gate is used to discriminate
single beads from double beads. A gate should be drawn on the
histogram only when the X axis is set to Doublet Discriminator.
gated event An event that falls within the user defined gate. The gate defines the
size of one bead, thus gated events are events that are the correct size
to be a bead.
histogram The representation of frequency distribution for analyte results. The
DD channel results are often displayed in a histogram format.
immunofluorescence A technique which uses a covalently linked fluorochrome-antibody
complex to detect or quantify a particular antigen.
kit A group of standards and controls used to create a protocol. They are
used in the protocol’s definition for the multi-analyte assays that will be
performed. Formerly called “product” in previous releases of Luminex
software.
Luminex xMAP microsphere set Luminex multi-analyte microspheres containing a unique mixture of
two distinctly colored fluorochromes to distinguish them from other