Luminex xPONENT 4.0 SP1 - Manual (Page 69)

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Touring the Software
FIGURE 38. Settings Tab
This tab contains the following:
Name and Description boxes. Type a name and a description in the appropriate boxes.
Acquisition Settings. Use this section to set the following:
• Bead Type - Select either MicroPlex (standard) or MagPlex (magnetic) beads.
• Volume - This is the volume the instrument will aspirate into the system for analysis.
Type the desired sample volume in microliters. Use values from 10 to 200 µl for both
96-well and 384-well plates. To avoid air intake, add at least 25 µl to the sample well in
addition to the sample size. The default value is 50 µl.
• Timeout - Select Enabled to enable a timeout. Acquisition stops once the timeout is
reached. Type a value from 1 to 250 seconds.
• XY heater - Select Enabled to enable the XY heater. In the box, type the desired value
in degrees Celsius. The temperature range is 35 to 60°C in 0.5 increments.
WARNING: Acquiring data before the heater has reached the proper
temperature can compromise test results.
• Plate Name - Click the Plate Name list and select Current 96-well plate, Current
384-well plate, or a plate that you have previously saved.
• DD gating - Use this to set the minimum and maximum DD gating values. Use values
within the range of 0 to 32767. In the first box, type the minimum value. In the second
box, type the maximum value. The default minimum and maximum values are 7500
and 13500 respectively.
• Reporter Gain - Select Standard PMT from this list to decrease the reporter gain
level. The default option is Enhanced PMT. Refer to assay application instructions for
more detailed information.