YSI ProODO User Manual

Page 15

display. Highlight the barometer value and press

enter to adjust it if needed. If the barometer

reading is incorrect, it is recommended that

you calibrate your barometer. Note - the

barometer should be reading “true” barometric

pressure (see Barometer section for more

information on “true” barometric pressure).

If the value is acceptable, there is no need to

change it or perform a barometer calibration.

Wait for the temperature and DO% values

under “Actual Readings” to stabilize, then highlight Accept Calibration and

press enter to calibrate. If User Field 1 or 2 are enabled, you will be prompted to
select the fields and then press Cal

to complete the calibration. The message

line at the bottom of the screen will display “Calibrating Channel...” followed by
“Calibration Successful”. Press Esc

to cancel the calibration.

CALIBRATING DO % IN AIR SATURATED WATER:

1-POINT CALIBRATION

Continuously sparge water with air using an air stone or some type of aerator

until the water becomes completely saturated with air. For 500 ml of water at

room temperature, it may take 60 minutes or more for the water to become

completely saturated

Place the sensor in the air saturated water, be sure to

immerse both the ODO and Temperature sensor into the water, and wait for the

readings to stabilize (at least 2 minutes).

Press Calibration

. If Probe ID or User ID are enabled in the System GLP

menu you will be able to highlight these features and add, select, edit, or delete

an ID. When enabled these IDs are stored with each calibration record in the

GLP file.

After selecting the Probe ID and/or User ID if appropriate, highlight DO and

press enter. Highlight DO % and press enter to confirm.

The instrument will use the value from the internal barometer and will display

this value in brackets at the top of the display. Highlight the barometer value

and press enter to adjust it if needed. If the barometer reading is incorrect,

it is recommended that you calibrate your barometer. Note - the barometer

should be reading “true” barometric pressure (see Barometer section for more

information on “true” barometric pressure). If the value is acceptable, there is

no need to change it or perform a barometer calibration.

Wait for the temperature and DO% values under “Actual Readings” to stabilize,

then highlight Accept Calibration and press enter to calibrate. If User Field 1
or 2 are enabled, you will be prompted to select the fields and then press Cal

to complete the calibration. The message line at the bottom of the screen will

display “Calibrating Channel...” followed by “Calibration Successful”. Press Esc

to cancel the calibration.

CALIBRATING IN AIR SATURATION PERCENT (DO %):

2-POINT CALIBRATION WITH ZERO SOLUTION

Place the sensor in a solution of zero DO. Be sure to immerse both the ODO and

Temperature sensor into the water.

A zero DO solution can be made by dissolving approximately

8 - 10 grams

sodium sulfite (Na

) into 500 mL tap water or DI water. Mix the solution

thoroughly. It may take the solution 60 minutes to be oxygen-free.

Press Calibration

. If Probe ID or User ID are enabled in the System GLP

menu you will be able to highlight these features and add, select, edit, or delete

an ID. When enabled these IDs are stored with each calibration record in the

GLP file.

After selecting the Probe ID and/or User ID if appropriate, highlight DO and

press enter. Highlight Zero and press enter. Wait for the temperature and

DO% values under “Actual Readings” to stabilize, then press enter to Accept

Calibration. If User Field 1 or 2 are enabled, you will be prompted to select the
fields and then press Cal

to complete the calibration. The screen will then

prompt for a follow-up second point calibration.

Highlight DO% for the second calibration point and press enter to continue with

the next point at full saturation. Rinse the sensor of any zero oxygen solution

using clean water. Follow the steps under either of the DO % Saturation methods

previously discussed to complete the second point.

CALIBRATING IN MG/L OR PPM AS A TITRATION:

1-POINT CALIBRATION

Place the sensor into a water sample that has been titrated by the Winkler method

to determine DO concentration. Allow the sensor to stabilize.

Press Calibration

. If Probe ID or User ID are enabled in the System GLP

menu you will be able to highlight these features and add, select, edit, or delete