Ocean Optics Overture - Manual (Page 50)

Appendix A: Sample Experiments
42 000-20000-400-01-201108
Fluorescence and Stokes shift
Objectives:
Using the side port of the sampling unit the Stokes shift of fluorescent compounds in solution can be
measured.
Procedure:
1. Using an open bench set up and a UV lamp you can detect fluorescence by pointing the fiber at the
side of the cuvette.
2. Visible fluorescence of fluorescein from UV excitation by a UV led can be measured. This
phenomenon is used by opticians to show up scratches on the cornea and is the basis of confocal
microscopy used to view biological samples.