Ocean Optics Overture - Manual (Page 33)

Chapter 4: Experiments
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Fluorescence
The sampling unit can be set up to measure fluorescence from a side port at 90 degrees to the light path.
Light coming from the sample at 90 degrees has either been scattered or is fluorescence from the sample.
The intensity of fluorescent light is much lower than the in-line light used for absorbance or transmission.
As a result, you need a fiber optic probe with a larger diameter to collect more light. The mirror also helps
to redirect light into the sample to increase the fluorescence.
The Fluorescence fiber (P400-2-VIS/NIR) can be obtained from your supplier.
The following spectra were taken from a fluorescein dye used in a domestic floor cleaner.