Ocean Optics Overture - Manual (Page 31)

Chapter 4: Experiments
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4. Choose Beer-Lambert Law or Calibration. To use the Beer-Lambert Law option you need to
know the molar absorption (extinction coefficient ε) for the compound you are using. If you are
trying to find ε you need to choose the option “Calibrate from solutions of known concentration.”
5. Wavelength range selection. Overture software needs to know which wavelength to measure for
absorbance. Select one (Lambda max) or a range around Lambda max. You can see the
absorbance spectrum behind the dialog box.