Ocean Optics Overture - Manual (Page 32)

Chapter 4: Experiments
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6. Create the calibration curve. Take at least three samples of known concentration. Either enter
the Absorbance by hand from your notes or scan for it using the Scan now button. When you
enter the third sample a regression line will be plotted. The line can be displayed in first or second
order and forced through the origin. Values for the molar extinction coefficient and R
2 are
calculated.
7. Concentration meter. Now you can place any unknown concentration of your compound into
the cuvette holder and Overture software will give you an instant reading of the concentration and
show where it sits on the calibration curve with a red diamond marker. No units are shown next to
the reading because you have to know what units you are working in. You can make notes in Step
6 of the units you are working in.