Ocean Optics Overture - Manual (Page 49)

Appendix A: Sample Experiments
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Spectrophototmetric analysis of a buffer
solution – Isobestic point
Procedure:
1. Measure and analyze the visible light absorbance spectrum of an acetate buffer solution containing
bromocresol green indicator.
2. Compare the spectra of a sample of acetate buffer that has been treated with acid to a sample of the
buffer treated with base.
3. Use test results to calculate the pH of the buffer solution.