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Autoclaving, Maintenance, Autoclaving the tubing – Gilson 402 User Manual

Page 89

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6-5

Maintenance

6

Autoclaving

Autoclaving

Certain parts of the 402 can be autoclaved (20 min.

at 0.1 MPa, 121° C). They must be removed from

the instrument for autoclaving.

The autoclavable parts are:
• The valve (disassembled) or the Tee-junction.

• The syringe (except the 25 mL syringe).

• The piston assembly.

• PTFE and FEP tubing.

Autoclaving the Tubing

The tubing can be cleaned or autoclaved.

This procedure prevents the end of the tubing from

deforming. It is only necessary for the first time you

autoclave the tubing.

Precautions to take when autoclaving the tubing:
1) To preserve the flanged end of the tubing,

perform the following steps for the first time

that you autoclave these components.

2) Insert and tighten the flanged end of the inlet

tubing into one end of the PVDF union connector.

Tighten the flanged end of the outlet tubing to the

other end of the PVDF union connector. Connect

the other end of the inlet tubing to the filter.

3) Autoclave the tubing while connected to the

union connector.

4) Disconnect the PVDF union connector.
5) Autoclave the tubing a second time to sterilize

the interior of the tubing.

Union
connector

Transfer
tubing

Tubing
connector

Seal
(threaded)