Gilson 402 - Manual (Page 81)

5-31
Operation - Advanced Use
5
Additional Information
Flow Rates
Flow rates depend on the nature of the liquid
(viscosity), the capacity of the syringe, and the
internal diameter of tubing. For liquids transferred
via the probe, it is possible to adjust the aspirate
and dispense flow rates according to the nature of
the liquid (e.g. viscous liquids must be aspirated
slowly). The default value for ‘Air flow rate’ is 4
ml/min. Whatever the capacity of the syringe(s).
In Configuration
In the configuration menus, you can modify the air
flow rate and the flow rates of liquids coming from
the Reservoir (Prime flow rate and Reservoir aspirate
flow rate).
In Methods (Tasks)
In the Flow menu of each task, you can adjust the
flow rates of liquid aspirated via the probe. Maxi-
mum values (see tables) are given according to
syringe capacity and internal diameter of the
tubing. These maximum values have been opti-
mized without air gap. When using air gap, it is
recommended that you decrease these values.
Flow rates may be optimized using in-run prompts:
when you enter a question mark in the place of a
value (in task edit), that value has to be validated
before the run. Optimization of flow rates is
essential to assure maximum rapidity and volu-
metric precision. The default values used by the
software are shown in bold in the tables that
follows.
When using Titrate or Measure, you are advised to
halve the maximum values shown in the tables.
Additional Information
Configuration Flow Rates
Syringe size Flow rate
(mL/min)
100 µL 4
250 µL 10
500 µL 20
1 mL 40
5 mL 120
10 mL 120
25 mL 120
Syringe Volume (µL)
Tubing Type 100 250 500 1000 5000 10000 25000
1 ml 4 10 20 40 120 120 120
2 ml 4 10 20 40 100 100 100
5 ml 4 10 20 40 80 80 80
10ml 4 10 20 30 30 30 30
Micro Tips (liquid) 4 10 20 40 120 120 120
Micro Tips (air) 4 10 20 40 120 120 120
Large Tips (air) 4 10 20 40 120 120 120
Maximum Aspirate Flow Rate
(No Air Gaps)