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Additional information, Operation - advanced use, Flow rates – Gilson 402 User Manual

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Operation - Advanced Use

5

Additional Information

Flow Rates

Flow rates depend on the nature of the liquid

(viscosity), the capacity of the syringe, and the

internal diameter of tubing. For liquids transferred

via the probe, it is possible to adjust the aspirate

and dispense flow rates according to the nature of

the liquid (e.g. viscous liquids must be aspirated

slowly). The default value for ‘Air flow rate’ is 4

ml/min. Whatever the capacity of the syringe(s).

In Configuration

In the configuration menus, you can modify the air

flow rate and the flow rates of liquids coming from

the Reservoir (Prime flow rate and Reservoir aspirate

flow rate).

In Methods (Tasks)

In the Flow menu of each task, you can adjust the

flow rates of liquid aspirated via the probe. Maxi-

mum values (see tables) are given according to

syringe capacity and internal diameter of the

tubing. These maximum values have been opti-

mized without air gap. When using air gap, it is

recommended that you decrease these values.
Flow rates may be optimized using in-run prompts:

when you enter a question mark in the place of a

value (in task edit), that value has to be validated

before the run. Optimization of flow rates is

essential to assure maximum rapidity and volu-

metric precision. The default values used by the

software are shown in bold in the tables that

follows.
When using Titrate or Measure, you are advised to

halve the maximum values shown in the tables.

Additional Information

Configuration Flow Rates

Syringe size

Flow rate

(mL/min)

100 µL

4

250 µL

10

500 µL

20

1 mL

40

5 mL

120

10 mL

120

25 mL

120

Syringe Volume (µL)

Tubing Type

100

250

500

1000

5000

10000

25000

1 ml

4

10

20

40

120

120

120

2 ml

4

10

20

40

100

100

100

5 ml

4

10

20

40

80

80

80

10ml

4

10

20

30

30

30

30

Micro Tips (liquid)

4

10

20

40

120

120

120

Micro Tips (air)

4

10

20

40

120

120

120

Large Tips (air)

4

10

20

40

120

120

120

Maximum Aspirate Flow Rate

(No Air Gaps)