Gilson 402 - Manual (Page 52)

5-2
Operation - Advanced Use
5
Correct Use of the 402
Correct Use of a 402
Single-syringe Model
• Samples and reagents are aspirated from the
probe side.
• Liquid aspirated from the probe side may not
enter the syringes. Thus, the tubing that connects
the syringe to the probe, called transfer tubing, has
to be carefully chosen to accommodate the
volumes of samples and reagents defined in the
Method.
• Liquids should be separated by an air gap to
minimize contamination.
• If a drop of liquid is present at the end of the
tubing after dispensing, you must include this
drop in the result vial.
• Syringe functionality is shown below.
From Reservoir
Dual-syringe Model
• Samples and reagents are aspirated from the
probe side.
• Liquid aspirated from the probe side may not
enter the syringes. Thus, the tubing that connects
the syringe to the probe, called transfer tubing, has
to be carefully chosen to accommodate the
volumes of samples and reagents defined in the
Method.
• Liquids should be separated by an air gap to
minimize contamination.
(a) Aspirating from Reservoir (b) Aspirating from Probe (c) Dispensing
From Probe To Probe
Piston descends
to aspirate Piston descends
to aspirate Piston ascends
to aspirate