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Correct use of a 402, Operation - advanced use, Single-syringe model – Gilson 402 User Manual

Page 52: Dual-syringe model

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5-2

Operation - Advanced Use

5

Correct Use of the 402

Correct Use of a 402

Single-syringe Model

• Samples and reagents are aspirated from the

probe side.

Liquid aspirated from the probe side may not

enter the syringes. Thus, the tubing that connects

the syringe to the probe, called transfer tubing, has

to be carefully chosen to accommodate the

volumes of samples and reagents defined in the

Method.

Liquids should be separated by an air gap to

minimize contamination.

• If a drop of liquid is present at the end of the

tubing after dispensing, you must include this

drop in the result vial.

• Syringe functionality is shown below.

From Reservoir

Dual-syringe Model

• Samples and reagents are aspirated from the

probe side.

• Liquid aspirated from the probe side may not

enter the syringes. Thus, the tubing that connects

the syringe to the probe, called transfer tubing, has

to be carefully chosen to accommodate the

volumes of samples and reagents defined in the

Method.

• Liquids should be separated by an air gap to

minimize contamination.

(a) Aspirating from Reservoir

(b) Aspirating from Probe

(c) Dispensing

From Probe

To Probe

Piston descends
to aspirate

Piston descends
to aspirate

Piston ascends
to aspirate