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Operation - advanced use – Gilson 402 User Manual

Page 72

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5-22

Operation - Advanced Use

5

Optionally, you can supply up to 12 reagents, press

Reag, key in number of reagents, press ENTER.
Press Next, then for each reagent, key in volume

press ENTER. After the data are entered the Reag

key changes to REAG.
Press ESC until you see this screen (three times if

you have added reagents)
Press ESC again to reveal the Save Screen; select

Yes, the Method is then saved, after which you see

the Run Screen.
Run Procedure
Press Run
Select the syringe (for the Dual-syringe 402 only),

press the Chge soft-key to select LEFT or RIGHT,

press ENTER to confirm.
You are advised to select the left-hand syringe if the

volume to be measured seems to be significantly

larger than the volume of the right-hand syringe.
The maximum volume that you can measure is

limited to the volume of the aspirating syringe or

the transfer tubing size, whichever is the smaller.
Press Next,
Key in the flow rate at which you want the volume

measurement to take place, and then press ENTER.

The default flow rate is half the maximum possible

for the combination of syringe and transfer in use

(refer to pages 5-31, 5-32).
Press Run, press the command button, aspirate the

air gap:
Insert the tip of the tubing into the liquid to be

measured press the command button once to start

the measurement and a second time to stop. Refer

to page 5-12 for details of the measuring process.

At the end, the measured volume is shown on the

screen.
At his point you can either aspirate diluent from

the reservoir or via the probe. When you aspirate

from the reservoir, the diluent volume and the

measured volume are chained together in the

transfer tubing and are dispensed together. If you

use the probe, or if reagents are added, the liquids

are dispensed in three steps:
(i) you dispense the measured sample,
(ii) you aspirate the diluent, reagents, and mea-

sured sample,

(iii) you dispense all of the above into a vial.

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