Gilson 402 - Manual (Page 72)

5-22
Operation - Advanced Use
5
Optionally, you can supply up to 12 reagents, press
Reag, key in number of reagents, press ENTER.
Press Next, then for each reagent, key in volume
press ENTER. After the data are entered the Reag
key changes to REAG.
Press ESC until you see this screen (three times if
you have added reagents)
Press ESC again to reveal the Save Screen; select
Yes, the Method is then saved, after which you see
the Run Screen.
Run Procedure
Press Run
Select the syringe (for the Dual-syringe 402 only),
press the Chge soft-key to select LEFT or RIGHT,
press ENTER to confirm.
You are advised to select the left-hand syringe if the
volume to be measured seems to be significantly
larger than the volume of the right-hand syringe.
The maximum volume that you can measure is
limited to the volume of the aspirating syringe or
the transfer tubing size, whichever is the smaller.
Press Next,
Key in the flow rate at which you want the volume
measurement to take place, and then press ENTER.
The default flow rate is half the maximum possible
for the combination of syringe and transfer in use
(refer to pages 5-31, 5-32).
Press Run, press the command button, aspirate the
air gap:
Insert the tip of the tubing into the liquid to be
measured press the command button once to start
the measurement and a second time to stop. Refer
to page 5-12 for details of the measuring process.
At the end, the measured volume is shown on the
screen.
At his point you can either aspirate diluent from
the reservoir or via the probe. When you aspirate
from the reservoir, the diluent volume and the
measured volume are chained together in the
transfer tubing and are dispensed together. If you
use the probe, or if reagents are added, the liquids
are dispensed in three steps:
(i) you dispense the measured sample,
(ii) you aspirate the diluent, reagents, and mea-
sured sample,
(iii) you dispense all of the above into a vial.
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