Hoefer SE900 - Manual (Page 7)

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Function and Description
The Hoefer ® SE900 vertical slab gel electrophoresis unit is intended
for the second dimension of 2D electrophoresis. It is designed for gel
systems which use a single buffer within the gel tank such as those
described by Laemmli. Both buffers used in the anodic and cathodic
chambers must be the same. Up to 6 second dimension gel separations
can be performed simultaneously.
The first dimension separation of 2-D protein electrophoresis should
be performed on Immobilized pH Gradient Gels, referred to as IPG
strips in this manual. The Hoefer IEF100 can be used to generate first
dimension separations in which the proteins are separated by pI. The
focused strips are transferred to the SE900 second-dimension slab gel
for size separation.
The SE900 is offered as a self cast gel system. The SE900 glass
cassettes are 28 cm wide and 21 cm in length producing gels 25 cm
wide × up to 20 cm tall, and 1 mm in thickness. The SE900 will also
hold glass plates and gels from other vendors.
The SE900 is the separation tank as a stand alone unit. The
SE900-1.0 comprises the separation tank, a multiple gel caster, and
six glass cassettes.
Fig 1. The Hoefer SE900.